2274 - Ongoing Supply of Molecular Biology Consumables
CPI require a number of molecular biology consumables to be used during their research and development work.
Buyer
CENTRE FOR PROCESS INNOVATION LIMITED
Value
£3,900,000
Published
25 Apr 2022
Closes
26 Nov 2021
Procurement details
Source
Find a Tender
Region
South Teesside
Procurement method
Open
Category
Goods
Contract starts
Not provided
Contract ends
Not provided
CPV classifications
24000000
Chemical products
Lots
Lot 1 Enzymes
£3,900,000
Ongoing supply of enzymes. Enzymes should be tested for exonuclease and endonuclease activity. All enzymes should be provided with the manufacturer's definition of a "unit" of enzyme e.g. "one U of enzyme is defined as the amount of enzyme required to digest xµg of DNA at 37°C in xx minutes in a total reaction volume of xxµl"
Delivery: United Kingdom
Lot 2 Nucleotides
£3,900,000
dNTPs and NTPs will be required for use in multiple PCR applications; in vitro transcription; mRNA/saRNA synthesis and cloning. dNTPs and NTPs should be possible of being supplied in sodium or tris salt form and confirmed as RNase, DNase and protease free. The ability to supply alternative salt forms, such as lithium, would be advantageous. These dNTPs and N...
Delivery: United Kingdom
Lot 3 Primers and DNA Oligos
£3,900,000
CPI requires the synthesis of primers, DNA oligos, and gene synthesis services. CPI would require random primer sequences and the ability to buy custom synthesised primers for use in PCR/qPCR and would also require custom primer/ oligo synthesis, CPI will provide the sequence information for these primers to be synthesised. We would require primers to be pro...
Delivery: United Kingdom
Lot 4 Gel Consumables
£3,900,000
CPI would require consumables for the analysis of proteins and nucleic acids by gel electrophoresis. Consumables required include gel loading dyes; RNA, DNA and protein ladders and gel extraction kits. RNA and DNA ladders should span a range of sizes, up to 9kb. Concentrated gel running buffers such as 10XTAE, 10XTBE and MOPS is advantageous. The supply of o...
Delivery: United Kingdom
Lot 5 Buffers
£3,900,000
Concentrated buffers and nuclease free water for use with their associated enzymes during reactions are required. Buffers may be used where specified by the manufacturer in applications which include restriction reactions and in vitro transcription reactions. Buffers must be tested for exonuclease and endonuclease activity and a CoA must be available for eac...
Delivery: United Kingdom
Lot 6 Competent Cells
£3,900,000
CPI would require competent cells to be supplied for use in cloning/sub-cloning, protein expression and gDNA/cDNA library production.
Delivery: United Kingdom
Lot 7 RNA & DNA
£3,900,000
CPI require the supply of RNA and DNA purification kits. RNA/DNA purification kits can be either gravity flow, vacuum assisted or spin columns and should cover small scale through to large scale. Multiple types of PCR kits would be required for use in development activities at CPI also, such as RT-PCR and RT-qPCR kits.
Delivery: United Kingdom
Lot 8 Media
£3,900,000
CPI require the supply of cell culture media to assist with the testing of therapeutic nucleic acids (DNA/RNA). Cell culture media and feeds are required, as either pre-made sterile solutions or as a powder.
Delivery: United Kingdom
Lot 9 CleanCap
£3,900,000
CPI require supply of the RNA co-translational capping agent, cleancap. The supplier must be able to supply various types of therapeutically relevant cleancap analogues, such as AU and AG (in addition to the methylated form).
Delivery: United Kingdom
Lot 10 Columns
£3,900,000
CPI require columns for use in packed column chromatography during the purification of in vitro transcribed mRNA.
Delivery: United Kingdom
Lot 11 AAV Material
Value not provided
CPI require AAV material as a control or standard for downstream and analytical assays such as ELISA and western blotting. This material would be used to check the validity of assays and act as a comparison to in-house generated samples. Further, the material would be used to develop and validate methods for different analyses prior to analysis of samples ge...
Delivery: United Kingdom
Lot 12 ELISA Kits
£3,900,000
CPI require the supply of ELISA kits for the detection of proteins during the production of different therapeutics such host cell protein contaminants, recombinant therapeutic proteins and AAV particles. A range of kits are required that target and confirm the presence of specific protein targets in samples. For AAV analysis, different serotypes exist, and s...
Delivery: United Kingdom
Lot 13 Reporter Assays
£3,900,000
CPI require reporter assays as an efficient method to measure cell health during experiments. Assays allow of aspects such as product related toxicity and detect differences in cell growth. A range of kits are required that allow real-time measurements, compatibility with manual handling and/or automation. Reporter assays are required to provide high sensiti...
Delivery: United Kingdom
Lot 14 Transfection Reagents
£3,900,000
CPI require the supply of transfection reagents to deliver nucleic acids in different formats (RNA/DNA) into cells for the testing or production of therapeutics.
Delivery: United Kingdom
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